Fermentation. The production of gas in the dough to render the bread
porous is known as fermentation. Both yeast and bacteria are used to pro-
duce gas, bacteria producing the gas in salt-rising bread, yeast in ordinary
bread.
Microorganisms in breadmaking. Fuller states that the "microorganisms
associated with the production of bread may be divided into three main
groups: (1) the yeast Saccharomyces cerevisiae, carefully purified and
selected strains of which are purposely added to the dough; (2) certain
bacteria, which, introduced either purposely or accidentally, assist in the
development of the gluten and give rise to characteristic flavoring products ;
and (3) the unwanted organisms, B. mesentericus, torulae, and moulds,
which are introduced chiefly from flour, air, and perhaps water, uninten-
tionally either into the dough or onto the baked loaf."
Fuller states that it seems likely that attention has been confined too
much to selecting and purifying yeasts in relation to their carbon dioxide
producing powers and that side reactions, i.e., bacteria-producing, essential
flavoring ingredients have been neglected. He cites the work of a Russian
microbiologist who examined the bacterial flora of a number of samples of
*'leaven." The dominant organism in all was Lactobacillus panis acidi,
with Streptococcus lactis acidi and others in small numbers. Adding Lacto-
bacillus panis acidi to yeast gave a bread with flavor superior to that of
bread in which yeast alone was used.
Enzyme action in fermentation. The yeast contains an enzyme, zymase,
that produces carbon dioxide and alcohol from the sugar. During fermenta-
tion many changes are occurring in the dough. The flour enzymes as well
as the yeast enzymes bring about definite reactions. The chief amylolytic
enzyme of the flour, diastase, produces maltose from starch. Flour contains
some initial sugar but this is used up in gas production and it is essential
for sugar to be formed for new gas production. Yeast also contains some
diastase. Sugar is usually added to bread dough. It is not essential, but
fermentation is more rapid. Most flours contain sufficient diastase for bread
making, but in some the diastatic activity is weak. Commercial bakers may
add diastase preparations to dough made of flours weak in diastase to
shorten the fermentation time.
Sugar is often added in rather large quantities in home-made bread and
it, plus the sugars already present in the flour, plus the sugar formed by
the diastase during fermentation, furnish the sugar for the action of the
zymase to produce carbon dioxide.
The proteinases hydrolize a part of the protein, and if very active may cause
harmful results. For, if the gluten, which during the first part of fermenta-
tion may be too tenacious, becomes too soft or thin the volume of the loaf
may be diminished and the texture impaired. Balls and Hale say a little
432 FLOUR AND BREAD
proteinase is beneficial. J0rgensen states that yeast water stimulates the
activity of proteinase.
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Fermentation. The production of gas in the dough to render the bread
porous is known as fermentation. Both yeast and bacteria are used to pro-
duce gas, bacteria producing the gas in salt-rising bread, yeast in ordinary
bread.
Microorganisms in breadmaking. Fuller states that the "microorganisms
associated with the production of bread may be divided into three main
groups: (1) the yeast Saccharomyces cerevisiae, carefully purified and
selected strains of which are purposely added to the dough; (2) certain
bacteria, which, introduced either purposely or accidentally, assist in the
development of the gluten and give rise to characteristic flavoring products ;
and (3) the unwanted organisms, B. mesentericus, torulae, and moulds,
which are introduced chiefly from flour, air, and perhaps water, uninten-
tionally either into the dough or onto the baked loaf."
Fuller states that it seems likely that attention has been confined too
much to selecting and purifying yeasts in relation to their carbon dioxide
producing powers and that side reactions, i.e., bacteria-producing, essential
flavoring ingredients have been neglected. He cites the work of a Russian
microbiologist who examined the bacterial flora of a number of samples of
*'leaven." The dominant organism in all was Lactobacillus panis acidi,
with Streptococcus lactis acidi and others in small numbers. Adding Lacto-
bacillus panis acidi to yeast gave a bread with flavor superior to that of
bread in which yeast alone was used.
Enzyme action in fermentation. The yeast contains an enzyme, zymase,
that produces carbon dioxide and alcohol from the sugar. During fermenta-
tion many changes are occurring in the dough. The flour enzymes as well
as the yeast enzymes bring about definite reactions. The chief amylolytic
enzyme of the flour, diastase, produces maltose from starch. Flour contains
some initial sugar but this is used up in gas production and it is essential
for sugar to be formed for new gas production. Yeast also contains some
diastase. Sugar is usually added to bread dough. It is not essential, but
fermentation is more rapid. Most flours contain sufficient diastase for bread
making, but in some the diastatic activity is weak. Commercial bakers may
add diastase preparations to dough made of flours weak in diastase to
shorten the fermentation time.
Sugar is often added in rather large quantities in home-made bread and
it, plus the sugars already present in the flour, plus the sugar formed by
the diastase during fermentation, furnish the sugar for the action of the
zymase to produce carbon dioxide.
The proteinases hydrolize a part of the protein, and if very active may cause
harmful results. For, if the gluten, which during the first part of fermenta-
tion may be too tenacious, becomes too soft or thin the volume of the loaf
may be diminished and the texture impaired. Balls and Hale say a little
432 FLOUR AND BREAD
proteinase is beneficial. J0rgensen states that yeast water stimulates the
activity of proteinase.